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Image Search Results
Journal: bioRxiv
Article Title: Secreted cytokines provide local immune tolerance for human stem cell-derived islets
doi: 10.1101/2022.05.09.487072
Figure Lengend Snippet: A. t-SNE projections of primary human islet cells. B. t-SNE projections of GAPDH expression across the assigned populations. Cells are colored according to their assigned cluster. (Adapted from Segerstolpe et al., 2016) C. Schematic of homology directed repair plasmids for integration of Luc2 and PD-L1 at the GAPDH locus. D. Schematic GAPDH-targeting Luc2 and peptide::B2M::HLA-E HDR plasmid. E. Schematic of the peptide::B2M::HLA-E long-chain fusion. F. PD1-Fc binding assay. PD-L1 and WT SC-islet cells were dissociated and stained with a 2-fold dilution series of PE-conjugated human and mouse PD1-Fc. Data is presented as MFI normalized to mode. G. FACS sorting strategy of HLA-ABC -/- hESCs. H. FACS gating strategy for quantification of Nkx6.1 + /C-pep + SC-β cells following in vitro differentiation.
Article Snippet:
Techniques: Expressing, Plasmid Preparation, Binding Assay, Staining, In Vitro
Journal: bioRxiv
Article Title: Secreted cytokines provide local immune tolerance for human stem cell-derived islets
doi: 10.1101/2022.05.09.487072
Figure Lengend Snippet: A. Quantification of SC-β cell survival when co-cultured with NK92mi cells. K562 and Raji cells were used as positive and negative controls, respectively. Cell survival is presented as mean ± SD (n = 5). B. NKG2A/NKG2C expression on NK92mi cells. C. FACS gating strategy of CD56 + NK cells enriched from human apheresis leukoreductions. Gates for specific population include all CD56 + NK cells (red), CD56 high (green) and CD56 dim (pink). Plots are representative of 5 donors. D. Quantitative analysis of CD56 expression on enriched primary human NK cells. Data is presented as % CD56 expression ( n = 5). E. In vivo NK cell assay. Bioluminescence imaging was performed on day 1 and 5 post-transplantation. F. Schematic of SC-endothelial cell differentiation protocol. G. FACS analysis of the endothelial cell marker CD31 in SC-endothelial cells derived from WT and B2M -/- hESCs.
Article Snippet:
Techniques: Cell Culture, Expressing, In Vivo, Imaging, Transplantation Assay, Cell Differentiation, Marker, Derivative Assay